n terminal his 6 affinity tag Search Results


human recombinant sirt3 containing a n-terminal hexahistidine tag  (Cayman Chemical)
90
Cayman Chemical human recombinant sirt3 containing a n-terminal hexahistidine tag
Human Recombinant Sirt3 Containing A N Terminal Hexahistidine Tag, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+his+6+affinity+tag/pmc05513672-145-5-11?v=Cayman+Chemical
Average 90 stars, based on 1 article reviews
human recombinant sirt3 containing a n-terminal hexahistidine tag - by Bioz Stars, 2026-08
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his6 mbp tev protease cleavage site sequence  (Addgene inc)
93
Addgene inc his6 mbp tev protease cleavage site sequence
His6 Mbp Tev Protease Cleavage Site Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
his6 mbp tev protease cleavage site sequence - by Bioz Stars, 2026-08
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gene synthesis  (GenScript corporation)
90
GenScript corporation gene synthesis
Gene Synthesis, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
gene synthesis - by Bioz Stars, 2026-08
90/100 stars
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n terminal his6 tagged acat1 plasmid  (Addgene inc)
92
Addgene inc n terminal his6 tagged acat1 plasmid
N Terminal His6 Tagged Acat1 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
n terminal his6 tagged acat1 plasmid - by Bioz Stars, 2026-08
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hexahistidine tagged yth domain  (Addgene inc)
93
Addgene inc hexahistidine tagged yth domain
Hexahistidine Tagged Yth Domain, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
hexahistidine tagged yth domain - by Bioz Stars, 2026-08
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pet28 vector (ndei/hindiii) encoding for an n -terminal hexahistidine tag  (GenScript corporation)
90
GenScript corporation pet28 vector (ndei/hindiii) encoding for an n -terminal hexahistidine tag
Structure of the BD-ObiF1 module. a ObiF1 from Burkholderia diffusa possesses a C-A-PCP-TE-MLP domain architecture. b Front and back side view of the holo-ObiF1 module. c The pPant arm is positioned in the C domain near the canonical catalytic C domain His residue (His144). Electron density (contoured at 3.0σ) calculated with mF o –DF c coefficients from an omit map (ligand occupancy set at zero) generated with simulated annealing. d A surface representation of the A and C domain interface illustrates the salt-bridging interactions that occur between the <t>N-terminal</t> region of the MLP domain and the A domain
Pet28 Vector (Ndei/Hindiii) Encoding For An N Terminal Hexahistidine Tag, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+his+6+affinity+tag/pmc06668435-315-27-31?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
pet28 vector (ndei/hindiii) encoding for an n -terminal hexahistidine tag - by Bioz Stars, 2026-08
90/100 stars
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oprd homologue  (ATCC)
99
ATCC oprd homologue
Structure of the BD-ObiF1 module. a ObiF1 from Burkholderia diffusa possesses a C-A-PCP-TE-MLP domain architecture. b Front and back side view of the holo-ObiF1 module. c The pPant arm is positioned in the C domain near the canonical catalytic C domain His residue (His144). Electron density (contoured at 3.0σ) calculated with mF o –DF c coefficients from an omit map (ligand occupancy set at zero) generated with simulated annealing. d A surface representation of the A and C domain interface illustrates the salt-bridging interactions that occur between the <t>N-terminal</t> region of the MLP domain and the A domain
Oprd Homologue, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
oprd homologue - by Bioz Stars, 2026-08
99/100 stars
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gst fusion tag  (GE Healthcare)
96
GE Healthcare gst fusion tag
Structure of the BD-ObiF1 module. a ObiF1 from Burkholderia diffusa possesses a C-A-PCP-TE-MLP domain architecture. b Front and back side view of the holo-ObiF1 module. c The pPant arm is positioned in the C domain near the canonical catalytic C domain His residue (His144). Electron density (contoured at 3.0σ) calculated with mF o –DF c coefficients from an omit map (ligand occupancy set at zero) generated with simulated annealing. d A surface representation of the A and C domain interface illustrates the salt-bridging interactions that occur between the <t>N-terminal</t> region of the MLP domain and the A domain
Gst Fusion Tag, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+his+6+affinity+tag/pmc07193511-111-32-17?v=GE+Healthcare
Average 96 stars, based on 1 article reviews
gst fusion tag - by Bioz Stars, 2026-08
96/100 stars
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histrap hp ni nta affinity column  (GE Healthcare)
97
GE Healthcare histrap hp ni nta affinity column
Structure of the BD-ObiF1 module. a ObiF1 from Burkholderia diffusa possesses a C-A-PCP-TE-MLP domain architecture. b Front and back side view of the holo-ObiF1 module. c The pPant arm is positioned in the C domain near the canonical catalytic C domain His residue (His144). Electron density (contoured at 3.0σ) calculated with mF o –DF c coefficients from an omit map (ligand occupancy set at zero) generated with simulated annealing. d A surface representation of the A and C domain interface illustrates the salt-bridging interactions that occur between the <t>N-terminal</t> region of the MLP domain and the A domain
Histrap Hp Ni Nta Affinity Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+his+6+affinity+tag/pmc03617296-353-13-18?v=GE+Healthcare
Average 97 stars, based on 1 article reviews
histrap hp ni nta affinity column - by Bioz Stars, 2026-08
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n-terminally tagged his6-sumo fusion protein  (GenScript corporation)
90
GenScript corporation n-terminally tagged his6-sumo fusion protein
Structure of the BD-ObiF1 module. a ObiF1 from Burkholderia diffusa possesses a C-A-PCP-TE-MLP domain architecture. b Front and back side view of the holo-ObiF1 module. c The pPant arm is positioned in the C domain near the canonical catalytic C domain His residue (His144). Electron density (contoured at 3.0σ) calculated with mF o –DF c coefficients from an omit map (ligand occupancy set at zero) generated with simulated annealing. d A surface representation of the A and C domain interface illustrates the salt-bridging interactions that occur between the <t>N-terminal</t> region of the MLP domain and the A domain
N Terminally Tagged His6 Sumo Fusion Protein, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+his+6+affinity+tag/pmc12141522-118-47-53?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
n-terminally tagged his6-sumo fusion protein - by Bioz Stars, 2026-08
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hexahistidine  (Addgene inc)
92
Addgene inc hexahistidine
Structure of the BD-ObiF1 module. a ObiF1 from Burkholderia diffusa possesses a C-A-PCP-TE-MLP domain architecture. b Front and back side view of the holo-ObiF1 module. c The pPant arm is positioned in the C domain near the canonical catalytic C domain His residue (His144). Electron density (contoured at 3.0σ) calculated with mF o –DF c coefficients from an omit map (ligand occupancy set at zero) generated with simulated annealing. d A surface representation of the A and C domain interface illustrates the salt-bridging interactions that occur between the <t>N-terminal</t> region of the MLP domain and the A domain
Hexahistidine, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+his+6+affinity+tag/pmc11171496-179-14-19?v=Addgene+inc
Average 92 stars, based on 1 article reviews
hexahistidine - by Bioz Stars, 2026-08
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tobacco etch virus tev protease cleavable n terminal his6 tag  (OriGene)
92
OriGene tobacco etch virus tev protease cleavable n terminal his6 tag
Structure of the BD-ObiF1 module. a ObiF1 from Burkholderia diffusa possesses a C-A-PCP-TE-MLP domain architecture. b Front and back side view of the holo-ObiF1 module. c The pPant arm is positioned in the C domain near the canonical catalytic C domain His residue (His144). Electron density (contoured at 3.0σ) calculated with mF o –DF c coefficients from an omit map (ligand occupancy set at zero) generated with simulated annealing. d A surface representation of the A and C domain interface illustrates the salt-bridging interactions that occur between the <t>N-terminal</t> region of the MLP domain and the A domain
Tobacco Etch Virus Tev Protease Cleavable N Terminal His6 Tag, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+his+6+affinity+tag/10__1021_slash_acs__jmedchem__5b01635-269-43-31?v=OriGene
Average 92 stars, based on 1 article reviews
tobacco etch virus tev protease cleavable n terminal his6 tag - by Bioz Stars, 2026-08
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Image Search Results


Structure of the BD-ObiF1 module. a ObiF1 from Burkholderia diffusa possesses a C-A-PCP-TE-MLP domain architecture. b Front and back side view of the holo-ObiF1 module. c The pPant arm is positioned in the C domain near the canonical catalytic C domain His residue (His144). Electron density (contoured at 3.0σ) calculated with mF o –DF c coefficients from an omit map (ligand occupancy set at zero) generated with simulated annealing. d A surface representation of the A and C domain interface illustrates the salt-bridging interactions that occur between the N-terminal region of the MLP domain and the A domain

Journal: Nature Communications

Article Title: The structural basis of N -acyl-α-amino-β-lactone formation catalyzed by a nonribosomal peptide synthetase

doi: 10.1038/s41467-019-11383-7

Figure Lengend Snippet: Structure of the BD-ObiF1 module. a ObiF1 from Burkholderia diffusa possesses a C-A-PCP-TE-MLP domain architecture. b Front and back side view of the holo-ObiF1 module. c The pPant arm is positioned in the C domain near the canonical catalytic C domain His residue (His144). Electron density (contoured at 3.0σ) calculated with mF o –DF c coefficients from an omit map (ligand occupancy set at zero) generated with simulated annealing. d A surface representation of the A and C domain interface illustrates the salt-bridging interactions that occur between the N-terminal region of the MLP domain and the A domain

Article Snippet: Genes encoding ObiF2 (WP_059467197.1), ObiH (WP_059467200.1), and ObiD (WP_059467195.1), were synthesized, codon optimized (Supplementary Figs. – ), and cloned into a pET28 vector (NdeI/HindIII) encoding for an N -terminal hexahistidine tag (Genscript; Piscataway, NJ).

Techniques: Residue, Generated